High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy
نویسندگان
چکیده
منابع مشابه
Large-volume en-bloc staining for electron microscopy-based connectomics
Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2012
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2011.439